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Briefly, according to the manufacturers protocol, GPX4-knockdown shRNAs were transiently transfected into Raji cells using Lipo8000 reagents (C0533, Beyotime)

Apoptosis Apoptosis is an extensively studied form of RCD that is orchestrated through the activation of caspases, protein cleavage, and the formation of apoptotic bodies

Bottazzo, G

[46] Glycogen and starch For details on the phosphorolytic degradation of starch and endogenous glycogen, refer to the dedicated sections

Measurement of ROS and Malondialdehyde (MDA) in liver tissues Homogenized liver tissues were mixed with 40 mM Tris-HCl buffer and centrifuged at 12,000 g for 10 min at 4C to obtain supernatant

DSIP promotes sleep in a species-specific manner, with varying effects on different types of sleep across species