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After blocking in 5% skimmed milk powder for 2 hours at room temperature, the samples were incubated overnight at 4C with primary antibodies, including ERK1/2 (#9102), p-ERK1/2 (#9101), JNK1/2 (#9252), p-JNK (#9251), p38 MAPK (#9212), and p-p38 MAPK (#9211), which were obtained from Cell Signaling Technology (Beverly, MA, USA), as well as VEGF-a (sc-7269) and Tubulin (sc-8035), which were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA)

They reemerged hepatocyte growth factor receptor (MET) signaling in LPS-induced CVX-BMDMs/RAW 264.7 cells, followed by activating downstream Ras pathway, which promoted the retrograde transfer of Arg-2 from mitochondria into the cytoplasm and remodeled energy/arginine metabolism by enhancing OXPHOS to mobilize M2 phenotype for improving rat osteoporotic bone defect (demonstrated by flow cytometry, IF, ELISA, and RT-qPCR) (Fig

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Oxidative stress-induced diseases via the ASK1 signaling pathway