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All above experiments were performed in triplicate

Heat control: If you are sensitive to spice, start with just a few dashes of Tabasco and work your way up

4 Conclusion The results of this study demonstrate that ChCl: glycerol based DES at a 1:3 molar ratio is highly effective for extracting protein from de-oiled soybean cake, offering a greener alternative to conventional alkali-acid methods

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