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To enhance myelin purity, crude myelin was resuspended in 0.32 M sucrose buffer and submitted to a second sucrose gradient centrifugation followed by 2 repeated osmotic shocks in hypotonic Tris-HCl buffer as described above

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[DOI] [PMC free article] [PubMed] [Google Scholar] Basu A, Du M, Leyva MJ, Sanchez K, Betts NM, Wu M, Aston CE, Lyons TJ

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