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In vitro scratch assays To assess the impact of PG/VG and nicotine on tumor cell migration, scratch assays were performed by adapting a protocol described elsewhere ( 4 cells were seeded into each side of 2-well coculture inserts containing a scratch or cell-free gap (80209, Ibidi) and incubated at 37C overnight
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Participants were prescribed varenicline at 1mg twice daily

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