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This allowed us to image and transform this networks onto binary, skeletonized shapes (Supplementary figure 2B-C), and describe them in terms of individualized or network-forming mitochondria, as well as quantify the percentage of image covered by DsRed2 signal (namely, mitochondrial footprint), the average length of network-forming branches and, finally, the average number of branches per mitochondrial network

The cigarette should be ashed whenever the ashes get to the length of about a 1/4" inch or more