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Cell viability assays were conducted using the Cell Counting Kit-8 (CCK-8) according to the manufacturer's instructions (Dojindo Laboratories, Kumamoto, Japan)

Using structured models such as the 5As (Ask, Advise, Assess, Assist, Arrange) or the Ask, Advise, Help framework ensures consistency and provides a patient-centered roadmap to support

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Vaping While smoking burns tobacco to create smoke that is inhaled exposing the lungs to tar, carbon monoxide, and thousands of combustion-related chemicals vaping does not burn anything

Chemical Pollution from Farming Tobacco farming uses pesticides and fertilizers that contaminate water sources