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For -actin, the forward primer was 5-TCA TGA AGT GTG ACG TGG ACA TC-3 and the reverse primer was 5-CAG GAG GAG CAA TGA TCT TGA TCT-3, with an annealing temperature of 60C
Alternative, smoke-free products that deliver nicotine without combustion and with far lower levels of toxicants than cigarettes are closer on the spectrum to cessation than combusted products (WHO, 2018
