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Chromatographic conditions There are several analytical methods available for measuring nicotine in cigarette.18-21 In the present study one of the published HPLC methods according to the laboratory condition was chosen and applied for measuring nicotine in cigarettes after some modifications.22 The HPLC system consisted of a pump (Model 600E, waters), a variable wavelength detector (Model 484, waters), a U6K injector and a recorder (Model 745B, waters)

Funding This work was supported by the National Heart, Lung, and Blood Institute (R01 HL112212 to DEL), the National Institute on Drug Abuse (K01 DA042687 to KPR, R01 DA050482 to KPR, and R01 DA054935 to DEL), and the National Institute on Aging (R01 AG069575 to EPH) of the National Institutes of Health, the Agency for Healthcare Research and Quality (T32HS000063 to JEB), and the American Lung Association (Innovation Award IA-834584 to KPR)

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