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Following this, two research assistants with prior coding experience of social media content, independently applied the codebook to 25 random posts and double coded them

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One primary endogenous source of ROS is the enzyme NADPH oxidase (NOX), which catalyzes the transfer of electrons from cytoplasmic NADPH to molecular oxygen, resulting in the formation of superoxide anions that react with NO to form peroxynitrite, a highly unstable species that can generate further ROS [21,22]

Our additional adjustment for the ratio of 3OH-cotinine/cotinine increased the model fit