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Then 40 g of homogenate was separated by polyacrylamide gel electrophoresis, transferred onto a nitrocellulose membrane [TGX precast midi protein gel (Biorad, Hercules, CA)], and incubated with primary and secondary antibodies directed against the proteins of interest
Am J Physiol Cell Physiol
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Biomarker studies suggest reduced exposure to some toxicants, but nicotine and carbon monoxide levels may not change significantly
Furthermore, it can detect outliers in the data automatically
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