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Cell pellets were suspended in 50 mM Tris-HCl buffer, pH 8.0, containing 4 mg/mL of MgSO 4 7H 2 O and 7.5 mg/mL of KCl and disrupted by sonication for 2 30 s with cooling

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However, there is major change in the retrohale, where a floral sweetness has replaced the raisin note from the first third, though the amount of white pepper remains about the same