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ZO-1 plays the critical function of anchoring this TJ protein to the cell cytoskeleton, thus allowing the cell to direct the distribution of these TJ proteins around the membrane [62]
It could indicate a more serious infection

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Some sticks start to drop off or get a bit flat toward the end of the 14-puff session, but Blue holds its profile pretty well all the way through
Other cards are located in places that may become unoccupied or even abandoned at certain points in the game
