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Moreover, smoking-related diseases, such as lung cancer, cardiovascular diseases, and respiratory disorders, have profound implications for healthcare systems worldwide

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Isolated RNA was normalized to 3.5 ng input/sample for automated library preparation with the Ion AmpliSeq Transcriptome Human Gene Expression Kit, using 12 cycles of amplification

There are 70 other known cancer-causing ingredients commonly found in most tobacco products

CRD42023385632) ( We followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, adhering to the 4-phase flow diagram and the 27-item checklist for this study (see PRISMA reporting checklist) 6