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Cells were lysed using microfluidization in 20 mM Hepes pH 7.4, 300 mM NaCl, 1 mM TCEP, and purified on an XK 50/20 column packed with ~250 ml of Ni Sepharose High Performance resin (Cytiva) followed by size exclusion using a HiLoad 16/600 Superdex 75 resin (Cytiva) column
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