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US pouch strengths ZYN: 3, 6, 9, 11 mgON!: 2, 4, 6, 8, 9 mgVELO: 3, 4, 6, 7, 9 mgRogue: 3, 6, 9, 12, 15 mgAlp: 3, 6, 9 mgZone: 3, 6, 9, 12 mgLucy: 4, 8, 12 mgSesh: 4, 6, 8 mgBlack Buffalo: 11.25 mgJuice Head: 6, 12 mgFRE: 3, 6, 9, 12, 15 mgZAR: 3, 6, 9, 16, 35 mg Want a lighter number

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The coverslips were then washed with PBS and the cells were fixed with 4% PFA for 15 min, washed with 0.05% Tween 20 (Promega, WI, USA) diluted in PBS (PBST), permeabilized with 0.2% Triton X-100 (Promega, WI, USA) for 10 min, and blocked with 3% BSA in PBST for 3 h

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