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Potent mutagens that are used in biomedical research include ethylmethanesulfonate and nitrosoguanidine

Individual Member States may also require manufacturers or importers to carry out studies as may be prescribed by the competent authorities in order to assess the effects of ingredients on health, taking into account, inter alia, their addictiveness and toxicity

The Ligero leaf must be blended carefully due its thickness and the lack of combustibility

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Most cigar leaf is cured this way as well, although it will also undergo an extra step, often referred to as "bulking" in which the leaves become fermented

Spatial, temporal, and demographic patterns in prevalence of chewing tobacco use in 204 countries and territories, 19902019: a systematic analysis from the Global Burden of Disease Study 2019