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thaliana demonstrated its effectiveness, using LshCas13a with crRNAs targeting TuMV helper component protease (HC-Pro) and GFP regions to reduce viral accumulation by up to 50% ( Figure 5 8.4 Bio-formulations Biological agents, which include polysaccharides, peptides and small proteins, and beneficial microorganisms, have reliably elicited or enhanced plant resistance against tobamoviruses, in part by priming innate immune pathways, altering host metabolism, and sometimes directly interfering with the virus (Trojak-Goluch, 2024)