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The cells were maintained in RPMI-1640 medium supplemented with 10% FBS, 100 U/mL penicillin, and 100 mg/mL streptomycin (Life Technologies/Gibco, Oshima, New York), in an incubator at 37 C and 5% CO 2

[9] Dopamine is involved in the brains reward circuit and is significant in the development of addiction

Smoking irritates the tissues in your mouth, leading to inflammation and increased sensitivity

CMAJ 191:E1321E1331 Goniewicz ML, Miller CR, Sutanto E et al (2020) How effective are electronic cigarettes for reducing respiratory and cardiovascular risk in smokers