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Insoluble material was removed by centrifugation at 30,000g for 20 min and the solubilized complex was immobilized by batch binding to M1 anti-Flag affinity resin in the presence of 5mM CaCl 2

Dr Ko Ho, director of the Gerald Choa Neuroscience Institute and an associate professor of CU Medicine, said the team made the discovery while pursuing effective interventions targeting ageing-related biological changes

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P = .028