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Archived from the original on June 13, 2013

This year, about 20 farmers are growing the new variety, and many of those who grew it last year have doubled their acreage this year and replaced acreage of less-sought-after chewing tobacco

Acknowledgements Research reported in this publication was supported by the Bill & Melinda Gates Foundation and Bloomberg Philanthropies

Quantitative real-time PCR Quantitative real-time PCR (qPCR) was conducted using the Applied Biosystems StepOne Real-Time PCR System and Fast SYBRGreen Master Mix (Applied Biosystems)

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Oriental tobacco leaves are harvested by hand, one by one, off of the plant as they ripen