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Others notice shortness of breath during moderate physical effort, such as climbing a few flights of stairs or walking briskly

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Still, he recalls that moment as one of the most nerve-wracking in his career: with his stomach in knots, he didnt sleep at all that night

"I would use food to regulate how I felt," he explained

The OAS was isolated from 5 g of wild-type TMV by RT-PCR in a reaction containing 0.2 M primer pLen_seq_rev, 200 U M-MLV Reverse Transcriptase RNaseH Minus Point Mutant (Promega, Walldorf, Germany), 1x M-MLV reaction buffer, and 1 mM dNTPs

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