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"Its actually showing them something they otherwise may not have been aware of until they started gaining all those wrinkles, that is actually happening to them and is going to happen to them, gradually, until they stop. Dr Becky Freeman, public health lecturer at the University of Sydneys School of Public Health said the study was interesting because it used appearance as a motivator instead of relying on health or financial motivators as has been done in the past

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RNA isolation and qRT-PCR Total RNA was extracted from cell lines with TRIzol Reagent (Invitrogen, CA, USA) following the manufacturers instructions, and was reverse-transcribed into cDNA, using M-MLV-RT and oligo(dT) primer (Invitrogen, CA, USA)

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