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Nicotine addiction, stress, a smokers belief that they will not be able to quit (self-efficacy), and the expectation that stop-smoking support is ineffective or will not meet their needs, time constraints or issues with access, have all been cited as barriers [10,11,12,13,14,15,16,17]

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The images were acquired by a Leica TCS SP8 X confocal microscope (Leica Microsystems, Germany) and the fluorescence intensity quantification was performed using a FiJi software 14