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The dehydrated biofilms were kept overnight in a vacuum oven at 25C, after which time they were sputter-coated with gold, examined, and imaged under a JEOL 6360 LV SEM (Soquelec, Montral, QC, Canada) operating at a 30 kV accelerating voltage

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To qualify as true HEPA, filters must remove 99.97% of particles at 0.3 micronsthe most penetrating particle size

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