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Typhimurium TA98 strain (1~2 10 9 cells/mL) was cultured in a sterile test tube for 12 hr, and 100 L of each sample was placed in a sterile cap tube, to which 500 L of S-9 mix was added for the metabolic activation-requiring of the indirect mutagen test

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Although this leaf is traditionally used to make chewing tobacco, snuff, snus, and in pipe blends

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It leverages the fact that lung cancer deaths reflect long, lagged smoking historiestypically cumulative exposure 2030 years earlier, although the lag is shorter at younger ages