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Reactions were performed in triplicate using the following conditions: one cycle at 95 C for 3 min, followed by 40 cycles at 95 C for 5 s and 60 C for 25 s, and a melting curve plot was constructed to confirm that no reaction produced non-specific amplification

Langmuir 1997, 13, 41504161

For example, if blunt use were automatically classified as tobacco co-use, 32.3% of students in this study who reported exclusively using tobacco-free blunt wraps would have been misclassified as using tobacco blunts

*Assumes average price of cigarettes at 11.94 per pack

If the temperature is advanced too slowly, sponging may occur

I detected notes of butter and wood, but they werent very deep