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This disruption can lead to decreased testosterone synthesis and secretion

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5 ) complex II-driven state III respiration (Rot), assessed in the presence of glutamate + malate + ADP + succinate + rotenone, and 6 ) CIV respiration (Asc + TMPD), where the link between the electron transport chain and ATP synthesis has been abolished, assessed by inhibiting complex III (antimycin A) and complex V (oligomycin) followed by the addition of TMPD + ascorbate

The effect of nicotine on energy expenditure during light physical activity