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Colonies were collected and cultured in 25 mL of brain heart infusion broth (Teknova, B9500, California, USA) supplemented with 1 mL of Fildes Enrichment (Remel, R45037, California, USA)

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Discussion KS is an autosomal recessive condition.5 While most reported patients are descendants of consanguineous parents (Cases 1 and 2), KS can also occur as a result of spontaneous mutation.10 While the parents of Cases 3 and 4 denied kinship, they both come from small border towns