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RIPA buffer was used for total protein extraction

Then I played the Brooklyn Zoo that summer and all the hoodlums came up
In addition to its known dehydration effects, some people report a sensitivity to propylene glycol which can lead to headaches

After washing cells thrice with PBS, for permeabilization, the cells were treated with 0.2% Triton X-100 at RT for 10 min

La vaporette dlivre de la nicotine travers un liquide chauff et inhal sous forme de vapeur
However, there are specific guidelines that need to be followed
