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Your diagnosis is Case 3 A 53 year old male who admits to social drinking as well as smoking one and a half packs of cigarettes a day, comes in on referral by his general dentist for the evaluation of a painless white area on the underside of his tongue

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Nicotine inhibits the production of proinflammatory mediators in human monocytes by suppression of I-B phosphorylation and nuclear factorB transcriptional activity through nicotinic acetylcholine receptor 7

The staining solution was aspirated and the cells were overlaid with 70% glycerol in PBS