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Possible side effects of the nicotine patch include: skin irritation such as redness or itching dizziness racing heart rate sleep problems or unusual dreams, which are more common with the 24-hour patch headache nausea muscle aches and stiffness Some side effects, such as a racing heart rate, could mean the nicotine dose is too high

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After incubation, cells were rewashed with PBS and imaged under a fluorescence microscope (Axiovert 40CFL with Axiocam MRm, Zeiss, Germany)