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smoking could not have harmful cumulative effects because each individual act had no short term consequences

Cells were first permeabilized using 0.1% Triton X-100 (Sigma-Aldrich, Germany) dissolved in PBS for 15 min at RT and then blocking buffer (2% BSA in sterile PBS) was added

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Sugiura T, Dohi Y, Hirowatari Y, Yamashita S, Ohte N, Kimura G, et al

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