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Protein extraction involves salting, heating, membrane filtration, and ion-exchange techniques, with detoxification steps to remove nicotine residues
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The different nicotine and nicotine salt groups showed a nonlinear curve characteristic of stronger DA with increasing concentration (Figures 2AG), and the slope factor of each fitted curve was statistically significant [Figure 2H, n = 3, one-way ANOVA, F ( p = 0.0009], indicating that each nicotine salt has a significant effect on the efficiency of concentration change to enhance the release of DA
The extract was prepared by treating the material for 15 h with 25% ammonia, followed by extraction with chloroform, acidbase liquidliquid extraction, and separation on a cellulose column (Lutomski 1960)
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