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Murine lung epithelial (MLE)-12 cells were exposed to cigarette smoke extract (CSE), and the molecular changes were evaluated

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(D) Western blots were performed, and (E) the relative protein levels of Sp1, SIRT1, acetyl-HIF-1, HIF-1, p21, and p27 in MLE-12 cells were determined (n = 3)

The second highest prevalence of smoking was evidenced in the group with a ninth- to 11th-grade education, with men outnumbering women by 27%