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The systematic review also relied on cohort studies, which may not detect less pronounced risks or risks that emerge over longer periods

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The investigators observed that the magnitude of the smoking effect seen in this younger cohort was greater than that found in cohorts older than 35 years of age studied elsewhere

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These effects are often seen as part of the cleansing process

For experimental metastasis assay, luciferase-labeled 4T1 cells (10 4 ) or E0771 cells (50 4 ) were injected into the tail vein of BALB/c or C57BL/6 mice followed by segregation into two groups as described in the spontaneous metastasis assay