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Among the flavouring chemicals tested, CAD was found to be the most toxic and O-vanillin and pentanedione also showed significant cytotoxicity

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The purified samples were analyzed by LC-MS/MS in selected reaction monitoring mode as previously described.25 PAH.Eleven PAH were analyzed using our previously described gas chromatography (GC)-MS method with slight modifications.26 Briefly,13 C-labeled internal standard mix was added to Cambridge filter pads which were then extracted in 12 mL cyclohexane on a benchtop shaker for an hour, followed by sonication for 10 min