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For one, governments in Asia are largely averse to having such discussions as the debate over the issue remains "polarised and emotional", said Professor Tikki Pangestu, a visiting professor at Lee Kuan Yew School of Public Policy and an expert in public health policies
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Finally, the optical density (O.D.) of each well was measured within 10 min using a microplate reader set to 450 nm, and then the concentration was calculated by comparison with a standard curve

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