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Once inhaled, reactive electrophiles, such as flavorings commonly used in e-liquids, activate TRPA1 by covalent modification of cysteine residues, altering protein complex conformation and facilitating inward flow of cations [116118]
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At the same time, nearly 10% of inhaled nicotine undergoes endogenous conversion to nitrosamine compounds such as nitrosonornicotine and nitrosamine ketone [96,97]
As soon as the pressure reached 80 PSI, the inflator shut off
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