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Five-hundred L supernatant was dried and reconstituted in 200 L isopropanol/acetonitrile/water (2:1:1 v/v/v), sonicated 10 min, centrifuged again and 150 L transferred to LC vials

The motivational valence of nicotine in the rat ventral tegmental area is switched from rewarding to aversive following blockade of the 7 subunit-containing nicotinic acetylcholine receptor

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Consistent with earlier findings, although the risk of death increased significantly with greater smoking intensity, it declined after cessation of smoking, the research team wrote