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IF microscopy (ALI wholemount IF labeling) ALI cultures on transwell inserts were rinsed with 1 PBS, fixed for 15 min in 3.2% PFA (no sucrose) at room temperature or 10 min in methanol at 20 C, rinsed twice more with 1 PBS and stored at 4 C
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Anti-inflammatory drugs (5-aminosalicylates, corticosteroids), immunosuppressants (azathioprine, mercaptopurines, cyclosporins), tumor necrosis factor inhibitors (Infliximab, adalimumab), anti-diarrheal, pain relievers, antispasmodics, and other medications are several of the treatment options for ulcerative colitis [48]

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