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In animal models, mice were exposed for 60 weeks to the e-cigarette with a concentration of nicotine from 0 to 24 mg/mL showed endothelial dysfunction and an increase in endothelial ROS, in addition to a thickening of the vessel wall were dependent on nicotine concentration (186)

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This lower nicotine level is a limitation of this study, especially as the nicotine levels of currently used e-cigarette disposables on the market are 50 mg/mL