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Methods Reagents Pharmaceutical grade glycerol (99.9% purity) was obtained from Sigma Aldrich (Gillingham, United Kingdom

Immunofluorescence staining and imaging Cells in AX12 were fixed using 4% paraformaldehyde in distilled PBS- (PBS without calcium and magnesium)

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10.5114/reum.2022.120752 209

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Furthermore, the interaction between social exclusion and brain regions highlights the nuanced representation of social influences and cognitive processes within different brain areas