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Ther Drug Monit (2011) 33(3):35561

treated cells were incubated with JC-1 dye (5 g/mL) for 30 min at 37 C, followed by fluorescence detection using a fluorescence microscope, where the red/green fluorescence ratio was calculated to quantify mitochondrial polarization status, as a higher ratio indicates healthier mitochondrial function while a lower ratio suggests mitochondrial depolarization and dysfunction

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A detailed description of the methods used to derive transition probabilities is provided in ESM Methods