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Standard qPCR techniques were followed (optimized for Roche LC480 Light Cycler) using QuantStudio 3 RT-PCR system as per the manufacturers instructions

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The federal National Institutes of Healths (NIH) NINDS aims to reduce the burden of neurologic disease by funding and conducting research on the nervous system

[**] OLS estimation produces similar estimates to the ML estimates presented here, however, ML estimation is preferred due to its robustness to distributional assumptions regarding the error term