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The specific antibodies used to detect skeletal muscle proteins included: oxidative phosphorylation complexes I-V (Total OxPhos, ab110411, Abcam, Cambridge, MA), Adenine Nucleotide Translocase-1 (ANT) (Ab110322, Abcam, Cambridge, MA), Uncoupling protein 3 (UCP3) (AB 3046, Milipore, Burlington, MA), Manganese-Dependent Superoxide Dismutase (MnSOD) (SC515068, Santa Cruz Biotech, Santa Cruz, CA), 4-hydroxynonenal (4-HNE) (ab46545

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One reason the levels of 1a-d are higher herein is that we used the harsher 50 puff replication study puffing regimen, which the researchers performing the latter study had modified 4 (without explanation) from our original milder 10 puff experiments

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