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The prepared samples were analyzed by liquid chromatography (LC)-tandem mass spectrometry (MS/MS) on a Hypercarb column (Thermo Scientific), using 10 mM ammonium acetate (with 0.01% formic acid) and methanol as mobile phase as previously described.23,24 Beta-carbolines.Harman and norharman were analyzed by using the same 10 mM ammonium acetate filter pad extracts that were prepared for nicotine and minor alkaloid analyses

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