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The recipient mice developed microbial imbalances similar to those observed in smoking mice and gradually gained weight, a phenomenon that was greatest in mice transplanted with microbiomes collected during the smoking cessation period

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A study published in JAMA Internal Medicine in 2014 concluded: "E-cigarette use by smokers was not followed by greater quitting, or reduction in consumption one year later." Another 2015 study in the journal Addiction found that daily use of e-cigarettes seemed to be associated with an increase in the rate of attempts to quit smoking and less smoking, though not increased rates of quitting