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Total genomic DNA was isolated using Abcam genomic DNA Isolation Kit (ab65358) according to manufacturer instruction

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Despite these problems, Project MIX results have been widely promoted to the scientific community [104][116], the public at large, and the Institute of Medicine [117], as well as cited by other tobacco industry scientists [118][122]

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Interestingly, those reporting headaches peaked on day three (72 hours) at 44%, dropped to 17% on day seven, and had declined to a low of just 11% on day fourteen