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We also made direct approaches to people in the relevant age range observed smoking (these approaches aimed to increase sample diversity) and used social media (Facebook) to elicit participants whose age, gender and ethnicity varied

"Pope Francis bans cigarette sales in the Vatican"

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Quantification of nicotine by mass spectrometry Nicotine was quantified from CSE by accurate mass measurement using LCMS with a synapt G2 Q-ToF mass spectrometer coupled to an Aquity chromatography system (Waters) using a method based on the work of McGuffrey et al

Kritz H, Schmid P, Sinzinger H (1995) Passive smoking and cardiovascular risk
There, it binds with hemoglobin, reducing your red blood cells ability to carry oxygen to other parts of the body